Purification and properties of a new DNase activity from KB cells
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Purification and properties of a new DNase activity from KB cells.
A deoxyribonuclease activity with specificity towards single-stranded DNA has been purified approximately four hundred-fold from KB cells, by chromatography on DEAE-cellulose, phosphocellulose and hydroxylapatite. The last step of the purification results in separation of the enzyme from a DNase activity which has been described previously (Wang, E.C., Furth, J.J. and Rose, J.A., (1978) Biochem...
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15 صفحه اولPurification and properties of nuclear and cytoplasmic deoxyribonucleic acid polymerases from human KB cells.
We describe the properties of three DNA polymerases isolated from nuclear and cytoplasmic fractions of freshly harvested KB cells. Two of the enzymes, cytoplasmic polylymerase and nuclear polymerase Nl, have been highly purified, one of them to apparent homogeneity. The third enzyme, nuclear polymerase N2, which is only partially purified, resembles the cytoplasmic enzyme in its physical proper...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1981
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/9.23.6635